Identification of Powdery Mildew Susceptibility Genes in Solanaceae PlantsJuly 1, 2015
Specific genes of the Mildew Locus O (MLO) gene family are linked to susceptibility towards powdery mildew fungal disease, which causes economic losses in agricultural settings. To search for source of powdery mildew resistance, Michela Appiano from Wageningen University and colleagues developed a breeding strategy based on the selective inactivation of MLO susceptibility genes in different plants.
PCR-based methodologies were used to isolate MLO genes from eggplant, potato, and tobacco, which are common hosts for powdery mildew fungi. The researchers were able to isolate the genes, namely SmMLO1, StMLO1 and NtMLO1 from eggplant, potato, and tobacco, respectively. Genetic analyses showed that these genes came from a common ancestor with tomato SlMLO1 and pepper CaMLO2, previously shown to be important for powdery mildew pathogenesis.
Furthermore, tobacco NtMLO1 was characterized as powdery mildew susceptibility gene. Single nucleotide mutation of NtMLO1 also led to complete loss-of-function of the gene.
The results of the study are vital in understanding MLO gene evolution in Solanaceae and for molecular breeding approaches to develop powdery mildew resistance using reverse genetics.
Read the research article published at Transgenic Research.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Tanzania Deputy Minister for Agri Advocates Adoption of Biotech in Agriculture
- Scientists Find New Tool for Pathogen to Pillage Plants
- Canadian Government Ratifies International Treaty on Plant Variety Protection System
- PhilRice Develops Method for Rice Viral Infection Detection
- Australian Farmers Planting More GM Canola
- Gene Associated with Morphine Production in Poppy Plant Discovered
- Rothamsted Research Releases Results of GM Wheat Field Trials
- Identification of Powdery Mildew Susceptibility Genes in Solanaceae Plants
- Host-Induced Gene Silencing Reduced Aflatoxin in Maize
- Mechanism Involved in Drought Response of Arabidopsis thaliana Identified
Beyond Crop Biotech
- Researchers Discover How Petunias Know When to Smell Good
- Gene Controls Behavioral Changes During Warmer Temperatures
From the BICs
- Journalists Applaud Increased Engagement with Agricultural Scientists
- Agriculture Boot Camp for Miss Uganda Contestants 2015/16
- Video: Where Do Farmers Get their Seeds?
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: