RNAi useful in Wheat Functional Gene Analysis
October 26, 2007| |
RNA interference (RNAi) is being used to determine gene functions in model plants like Arabidopsis and rice. However, its use in polyploid species (those with more than two sets of chromosomes) is still in the early stage. Nonetheless, it is being used in polyploid wheat since a single RNA construct can silence multiple copies of homologous genes. A new review published by the journal Transgenic Research discusses the utilization of RNAi in wheat functional gene analysis.
To date, RNAi has been used to target a number of genes like those coding for transcription factors, enzymes necessary for starch synthesis, and signal and storage proteins. Wheat varieties with accelerated flowering time, reduced amylase content and delayed senescence have been previously produced by RNAi. RNAi response has been documented in different tissues and developmental stages. It has also been shown to be stably inherited and sequence specific. One of the limitations of RNAi in wheat is targeting genes with conserved domains and duplications in the genome, as there is a high chance of silencing unwanted genes.
Read the abstract at http://www.springerlink.com/content/vm186p3212231m72/?p=0216c5ffb83f4014ab9db6efbfaac6a6&pi=0 or the full paper at http://www.springerlink.com/content/vm186p3212231m72/fulltext.pdf
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