Comparison of Agrobacterium and Particle Bombardment for Dev't of High-expressing, Low-copy Transgenic Plants
February 6, 2013| |
Scientists at the University of Queensland in Australia conducted a study to compare two mechanisms used in genetic transformation of plants, the Agrobacterium-mediated transformation (AMT) and particle bombardment using whole plasmid (WP) and excised minimal cassettes (MC). They aimed to compare the two in terms of transformation efficiency, transgene integration complexity, and transgene expression in plants.
For direct comparison, the researchers used identical expression cassette for the linked selectable marker and reporter genes in all treatments. Results showed that there were no significant differences in the transformation efficiencies of WP and MC when equal amounts of DNA were delivered. When the MC concentration was decreased, the transformation efficiency became equivalent with ATM, and thus both can be used efficiently to generate sugarcane lines with low transgene copy numbers and strong transgene expression.
Read the research article at http://link.springer.com/article/10.1007/s11248-012-9639-6#page-1.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Global Partnership to Boost Africa's Ag Research
- US-Indian Project to Develop Improved Pigeonpea Cultivars
- Burkina Faso's Cotton Output Increases by 57% due to GM
- Tanzania Hosts Key International Forum on Plant Viral Diseases
- Ghana's Minister Suggests the Use of GMOs to Boost Country's Food Security
- Nigeria to Host UNN Biotechnology Summit
- Research Shows New Means to Boost Maize Yields
- Costa Rica Approves GM Corn Cultivation
- Draft of Chile Genomic Sequence Completed, Hope to Improve Chile Breeds
- Two Seasons of Golden Rice Trials in the Philippines Completed
- Iloilo Agri Extension Officers Get Updates on Crop Biotech
- Philippine Policy Makers Recognize Local Biotech Researches and Products
- Survey on Extension Officials' Awareness on Bt Brinjal
- Students Visit Biotech Institutes in Bangladesh
- Korea Approves GM Corn Stacks for Importation
- GM Tobacco Produces Antibodies for Possible Rabies Treatment
- BASF Stops EU Approval Bid for GM Potatoes
-
Research Highlights
- Comparison of Agrobacterium and Particle Bombardment for Dev't of High-expressing, Low-copy Transgenic Plants
- Expression of Aquaporin gene in Salt-stressed Rice Pre-treated with Dopamine
-
Beyond Crop Biotech
- RNA Fragments From Microvesicle Can be Used for Cancer Diagnosis
- Manipulation of Mosquito Gut Bacteria to Fight Mosquito-Borne Diseases
-
Resources
- Developing Soybean Cultivars, a Video Presentation
-
Read the latest: - Biotech Updates (April 30, 2025)
- Gene Editing Supplement (April 30, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet