Introgression of Brassica rapa subsp. sylvestris Blackleg Resistance into B. napus
June 15, 2012| |
Blackleg is one of the predominant diseases of canola (Brassica napus). It is caused by a fungal pathogen called Leptosphaeria maculans. To address this problem, Fengqun Yu from Agriculture and Agri-Food Canada and other researchers transferred two blackleg resistance genes (LepR1 and LepR2) from B. rapa subsp. sylvestris (BRS) into canola through interspecific hybridizations. They analyzed the microsatellite markers in two backcross populations (WT3BC1 and WT4BC1) which showed that segregation fit a 1:1 ratio for BRS and non-BRS alleles.
The team used two L. maculans isolates (WA51 and pl87-41) to differentiate plants carrying resistance genes LepR1 and LepR2. They found that only 4.0 and 16.6% of the plants were resistant to isolates WA51 and pl87-41, respectively, in the WT3BC1 population, while 17.9 and 33.3% of the plants were resistant to these isolates, respectively, in the WT4BC1 population. Based on cotyledon resistance and marker-assisted selection (MAS), BC1 plant WT4-4, which carried a resistance gene similar to LepR1 (designated as LepR1′) and BC2S1 plant WT3-21-25-9, which carried LepR2′, were identified.
The resulting plants were successively backcrossed with B. napus. They used MAS in every generation to decrease non-resistance alleles related to the BRS genome and to recover the complete complement of C-genome chromosomes. This led to the formation of highly blackleg resistant B. napus lines.
The research paper is available at http://www.springerlink.com/content/b37u344048j14716/.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Dr. Daniel Hillel Named 2012 World Food Prize Laureate
- World Food Stocks Rise but Hunger Threatens Arab Countries
- SAVE Food Initiative to Reduce Wastage and Loss
- Global Seeds Market Report 2012
- Togo's Ministry of Agriculture Foresees Great Potential in Agricultural Biotechnology
- Ghana to Revive Cotton Production with Modern Biotech
- Vita Launches Potato Centre of Excellence in Africa
- GM Soybean Approved for Commercial Use in Mexico
- Current Challenges and New Tools to Combat Herbicide Resistant Weeds
- Overcoming Build-up of Insect Resistance Against Bt
- SIUE Makes Biofuel Breakthrough
- BASF Presents Innovations in the Pipeline
- SG Biofuels New Genomics Research Center to Support Commercial Advancements
- Next-generation Sequencing Technology Opens Doors to Discoveries
- GM Crops Shrink Farming's Pesticide Footprint
- Pakistan Minister Sees Need to Redefine Parameters of Agric Production
- Defense Mechanism of Lectin in Plant Uncovered
- Implications of GM Seeds on Chinese Farmers' Rights to Food
- Program on Development of Biotechnology in Russia Through 2020
- Rothamsted and BBSRC Launch "20:20 Wheat"
- New Insights on How Plants Fight Diseases
-
Research Highlights
- Scientists Trace Footprints of Photoperiod Pathway Genes in Oryza
- Introgression of Brassica rapa subsp. sylvestris Blackleg Resistance into B. napus
- Comparison on Mutagenic Effects of Commercial Soybeans in Brazil
-
Beyond Crop Biotech
- University of California Researchers Create Mosquitoes that Cannot Transmit Malaria
- GE Diamondback Moth to Decrease the Pest's Population
- Scientists Use Nanomedicine to Combat Herpes
-
Announcements
- Raman Fellowships for African Researchers in India
- 2012 Global Farmer's Roundtable Online Nominations Open
-
Read the latest: - Biotech Updates (May 2, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet