Targeted Modification of Rice Genome with Base EditingNovember 23, 2022
Experts from the Chinese Academy of Agricultural Sciences developed an experimental protocol for targeted modification of the rice genome using base editing, which is published in STAR Protocols.
Based editing is one of the approaches used for genome editing to generate single-nucleotide variants (SNVs) in genomes. The protocol presented is specifically for targeted adenine to guanine substitution in rice using an adenine base editor. A detailed design of the sgRNA, CRISPR plasmid construction, rapid genetic transformation of rice, and genotyping of editing events are discussed. It can also be used for cytosine base editing.
Download the complete document from STAR Protocols.
You might also like:
- Precise Base Editing of Acetolactate Synthase Genes Confers Herbicide Resistance in Maize
- Scientists Use CRISPR-Cas9 for Single-nucleotide Editing in Rice
- High-Efficiency Reduction of Rice Amylose Content via CRISPR-Cas9-Mediated Base Editing
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
Genome Editing Supplement (November 23, 2022)
Research and Tools
- Targeted Modification of Rice Genome with Base Editing
- Tomatoes Can Now Come in Customized Colors Thanks to CRISPR
- Scientists Use Virus for Bacterial Base Editing
- Study Finds CRISPR-Cas9 Leads to Unexpected Genomic Changes
- Scientists Report First use of CRISPR to Substitute Genes to Treat Patients with Cancer
Public Acceptance and Engagement
- Large Chinese Seed Companies Likely to Produce Gene-Edited Crops for Farmers - Study
Read the latest:
- Biotech Updates (March 29, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: