Precise Base Editing of Acetolactate Synthase Genes Confers Herbicide Resistance in MaizeJanuary 15, 2020
Researchers from Chinese Academy of Agricultural Sciences and other institutions reported precise based editing of maize genes using CRISPR-Cas9, leading to development of sulfonylurea herbicide resistance. The results are published in The Crop Journal.
A CRISPR-Cas9 nickase-cytidine deaminase fused with uracil DNA glycosylase inhibitor was used for targeted conversion of cytosine (C) to thymine (T) in two non-allelic acetolactate synthase genes (ZmALS1 and ZmALS2) to generate sulfonylurea herbicide resistant mutants. The results showed that both protoplasts and recovered mutant plants exhibited the activity of the cytosine base editor. Transgene-free edited plant with ZmALS1 mutation or a ZmALS1 and ZmALS2 double mutation were tested for their resistance at a dose of up to 15-fold the recommended limit of sulfonylurea herbicide.
Based on the results, the confirmed targeted base editing in maize plants exhibiting herbicide resistance could be a potential technique in precision breeding of maize.
For more details, read the research article in The Crop Journal.
You might also like:
- Pocket K No. 10: Herbicide Tolerance Technology: Glyphosate and Glufosinate
- Commercial GM Trait: Herbicide Tolerance
- GM Events with Sulfonylurea herbicide tolerance
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- USDA Reports Increase in Corn and Soybean Yields Amidst Challenging Weather
- International Consortium Sequence Canola Genome
- US Observes National Biotech Month, Launches Joint USDA-FDA-EPA Website for Biotech Regulation
- Research Identifies Protein for Plant Growth
- Researchers Move Closer to Developing Nitrogen-Fixing Cereal Crops
- Int'l Team Uses Genomic Data to Show How Plants Adapt to Changing Climate
- VIB-IPBO Offers Summer Course on Modern Breeding Techniques for Maize Improvement
- Two-week Old Corn Seedlings' RNA Data Produce Reliable Yield Predictions
Plant Breeding Innovations
- Genome Studies Show Genetic Variation of Lint Yield Components under Salty Field Conditions in Cotton
- Precise Base Editing of Acetolactate Synthase Genes Confers Herbicide Resistance in Maize
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: