Researchers Characterize Brassinosteroid Biosynthesis Genes in Rice
October 5, 2022
Nanjing Agricultural University researchers used CRISPR-Cas9 gene editing technology and characterized functional brassinosteroid biosynthesis genes in rice. The findings of their study are published in Plant Science.
CONSTITUTIVE PHOTOMORPHOGENIC DWARF (CPD) is a vital component in the brassinosteroids (BRs) biosynthesis pathway. Previous studies have shown that Arabidopsis has a single CDP gene, while two CPDs (OsCPD1 and OsCPD2) are present in the rice genome. However, no genetic evidence has been found about the requirement of OsCPD1 and OsCPD2 in rice BR biosynthesis. This led the researchers to define the role of OsCPD genes in BR formation.
The researchers constructed single and double mutants of OsCPD genes using the CRISPR-Cas9 gene editing tool. Results showed that double mutants develop multiple and obvious brassinosteroid-related defects. Furthermore, overexpression of OsCPD1 and OsCPD2 led to a typical brassinosteroid enhanced phenotype.
The findings provide genetic evidence that OsCPD1 and OsCPD2 play essential and redundant roles in maintaining plant architecture by modulating BR biosynthesis in rice.
Read more from Plant Science.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- Rice Plants Engineered for Better Photosynthesis Make More Rice
- Golden Rice Gets Approval from US FDA
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
Genome Editing Supplement (October 5, 2022)
-
Research and Tools
- Researchers Characterize Brassinosteroid Biosynthesis Genes in Rice
- ISAAA and Partners Call for Responsive Regulation of NBT Products
- Gene Editing to Help Curb Sterility Paradox in Fish Production
- CRISPR System Successfully Edits Fusarium Species
- Scientists Describe Type IV CRISPR-Cas System
-
Public Acceptance and Engagement
- EU Consultation Confirms Europeans' Support for Policy Change on Novel Genomic Techniques
-
Read the latest: - Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
-
Subscribe to CBU: - Share
- Tweet