All-in-one Plasmid CRISPR-Cas9 System Allows Rapid Genome Editing of B. subtilisSeptember 21, 2022
The CRISPR-Cas9 system has previously been found suitable for Bacillus subtilis, a microorganism commonly used in industrial enzyme production, but is limited by the selection of knockout genes, long editing cycle, and instability. Scientists have overcome these with the construction of an all-in-one plasmid CRISPR-Cas9 system which significantly improved the bacterium's transformation efficiency.
The new system achieved 100% efficiency for single gene deletions and point mutations, 96% efficiency for gene insertions, and at least 90% efficiency for plasmid curing. The system also completed one round of genome editing in just 2.5 days, continuously knocking out two extracellular protease genes. The engineering strain was also used to express Douchi fibrinolytic enzyme DFE27, and its extracellular enzyme activity reached 159.5 FU/mL
This rapid all-in-one plasmid CRISPR-Cas9 system, which only requires one plasmid transformation and curing, is the fastest iterative genome editing system for B. subtilis to the best of the developers' knowledge.
Read the full paper in Microbial Cell Factories.
You might also like:
- Engineered Bacteria Produces BeeFree Honey
- Genome Analysis of a Plant Growth-Promoting Bacillus
- GM Approval Database: Gene - cspB
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
Research and Tools
- KIT Researchers Use CRISPR to Prevent Genetic Exchange
- CRISPR Boosts Production of Medical Compound in Chicory
- Chromosome Engineering Now Possible in Mammals
- All-in-one Plasmid CRISPR-Cas9 System Allows Rapid Genome Editing of B. subtilis
Policy Considerations and Approvals
- EU Agriculture Ministers Agree on Plant Breeding Innovation to Strengthen Food Security and Sustainability in the Region
Public Acceptance and Engagement
- Textbook-style CRISPRpedia Now Available
Read the latest:
- Crop Biotech Update (September 28, 2022)
- Genome Editing Supplement (September 21, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: