Physical Model Maps Cas9 Cutting BehaviorMarch 23, 2022
Researchers from Delft University of Technology (TU Delft) have developed a physical-based model that establishes a quantitative framework on how CRISPR-Cas9 works, and allows them to predict where, with what probability, and why targeting errors (off-targets) occur.
A research team led by Martin Depken at TU Delft's Department of Bionanoscience shows how the new, physical-based model greatly improves existing models not only by predicting where the DNA is likely to be cut but also with what probability this will happen. Depken's team used a physics-based approach.
“In gene editing, you want to maximize the probability of cutting at the intended site, while minimizing the amount of cutting in the rest of the genome," Depken said. They created a model that can do this. He added that their model "changes the way in which to describe the gene editing from a binary choice to a complete probabilistic picture."
For more details, read the article on the TU Delft website.
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