Experts Develop PiggyBac for Delivery of CRISPR Tools into Stem CellsMarch 23, 2022
A group of experts from Pennsylvania State University and partners developed a technique that enhances the lifespan and efficiency of CRISPR gene-editing tools after delivery into stem cells. Bioactive Materials published their findings.
Stem cells are used by researchers to investigate the impact of disease on various tissue types due to their pluripotency or the ability to produce cells of any time. However, the delivery of gene-editing tools into stem cells can be inefficient, time-consuming, or expensive. Thus, the research team designed a delivery system using an enzyme that would allow for long-term integration of gene-editing tools.
Through conventional methods, the DNA components that transport tools into cells can last up to three days before being destroyed. With the enzyme in the system called PiggyBac, there will be permanent establishment of the editing tools into the cell's genetic code. The system was tested in human embryonic stem cells and it was shown that PiggyBac successfully and permanently integrated the gene editing tools into the cell's genome.
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