CRISPR-Cas9 Used to Confer Sulfamethoxazole Resistance in ArabidopsisOctober 20, 2021
A research team at Chonnam National University (CNU) used CRISPR-Cas9 to confer herbicide resistance in Arabidopsis by targeting the 5-oxoprolinase 1 (OXP1) gene. The results are published in Plant Biotechnology Reports.
Most of the herbicide tolerant crops developed using gene editing techniques have adopted site-specific mutations of endogenous genes. In the CNU study, the researchers aimed to confer sulfamethoxazole (Smex) herbicide resistance through insertion and deletion mutations using CRISPR-Cas9 editing of the OXP1 gene in Arabidopsis. Results showed that the mutants have reduced sensitivity to Smex. Furthermore, OXP1-edited plants were successfully obtained by screening the transgenic plants on Smex-containing media, implying the potential use of OXP1 as a marker for plant gene editing. The oxp1/CRISPR plants also exhibited tolerance to heavy metals, such as cadmium, and another sulfonamide compound, amisulbrom.
Based on the findings, the authors concluded that generating insertion-deletion mutations can be a potential technique to confer herbicide resistance in plants.Read the original article in Plant Biotechnology Reports.
You might also like:
- Plant Breeding Innovation: CRISPR-Cas9
- Herbicide Tolerance Technology: Glyphosate and Glufosinate
- Nagoya University Develops Highly Efficient CRISPR-Cas9 Vector for Arabidopsis
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- World Food Day 2021 Honors Food Heroes
- UN Biodiversity Conference Intensifies Call to Bend the Curve of Biodiversity Loss
- Research Team Engineers Bioenergy-Friendly Fungi
- Researchers Discover Gene for Improved Tomato Flavor and Shelf-life
- Pakistan Launches Digital Initiative to Empower Farmers
- Scientists Identify Biotech Techniques to Improve Space Agriculture
- Invitation to the 4th Asian Short Course on Agri-biotech, Biosafety Regulation, and Communication (ASCA2021)
- Golden Rice Favored as It Tastes, Smells like Regular Rice, but More Nutritious
- Breakthrough to Help Coconut Propagation and Conservation
- No Difference Found in Meat, Milk From Gene Edited Cows vs Conventional Cows
- CRISPR-Cas9 Used to Confer Sulfamethoxazole Resistance in Arabidopsis
- Japan Begins Sale of Genome-Edited "Madai" Red Sea Bream
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: