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Crop Biotech Update

TaDof1 Improves Nitrogen and Carbon Assimilation Under Low-Nitrogen Conditions in Wheat

April 28, 2021

Researchers at Forman Christian College University Lahore have discovered that the transcription factor TaDof1 improves nitrogen and carbon assimilation under low nitrogen conditions in wheat.

Wheat Dof1 (TaDof1) transcription factor is mainly associated with improved nitrogen assimilation in plants and taking this as a base this study has been conducted to develop a wheat variety with improved nitrogen and carbon assimilation under low Nitrogen conditions. After 4 weeks of Nitrogen stress in T1 plants, a substantial rise in the expression of citrate synthase (CS), isocitrate dehydrogenase (ICDH), phosphoenolpyruvate carboxylase (PEPC), and pyruvate kinase (PK) genes regulated by TaDof1 was observed. The findings of the study indicate a cooperative modification of nitrogen and carbon metabolisms in the transgenic variety since they are intimately linked together and the overexpression of TaDof1 in wheat resulted in a significant increase in various agronomic traits. The results clearly outline the merits of employing transcription factors in engineering plant metabolisms. They have also studied the effect of TaDof1 expression on physiological and biochemical parameters.

Read the research article in Plant Molecular Biology Reporter.

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