Genome Editing in Plants using CRISPR Type I-D NucleaseNovember 11, 2020
The researchers particularly used a type I-D (TiD) CRISPR-Cas genome editing system, which lacks the Cas3 nuclease domain but has Cas10d as its functional nuclease. Results showed that the mutations induced using TiD were different from those of CRISPR-Cas9 wherein both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, it was confirmed that TiD can be applied to generate bi-allelic mutant plants in the first generation.
Based on the findings, the researchers concluded that TiD is a unique CRISPR system that is applicable in plant genome editing.
Read more from Nature.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- CRISPR Technique 90% Effective in Reducing SARS-CoV-2 Coronavirus
- Tungro-Resistant Rice Developed through CRISPR-Cas9
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Study Identifies "Mother" of all SARS-CoV-2 Genomes; Reveals Earlier COVID-19 Timeline
- Researchers Find "Hidden Gene" in COVID-19 Virus
News from Around the World
- International Online Discourses Provide Global Updates on Animal Gene Editing and Biotechnology
- UN Report Pinpoints Potential Hotspots of Famine
- GM Crops to Boost Food and Nutrition Security in Ghana
- IITA and CIP Awarded Certification in Stewardship Excellence
- 30 Years of Experiments Predict Future for Major Crops
- South Australia Rejects 11 Proposals to Ban GM Crops, Says GM Moratorium's Price Premiums Only a Myth
- Australian OGTR Issues License for Commercial Release of Herbicide Tolerant Cotton
- Invitation to the 3rd Asian Short Course on Agri-biotech, Biosafety Regulation, and Communication
- Cisgenic Potatoes Do Not Disrupt Soil Microsystems
Plant Breeding Innovations
- Genome Editing in Plants using CRISPR Type I-D Nuclease
- Heat Tolerance Gene in Corals Found Using Gene Editing
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: