Tungro-Resistant Rice Developed through CRISPR-Cas9April 11, 2018
Rice tungro disease (RTD) is a serious threat in rice production. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus (RTBV). Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). To develop new sources of resistance to RTD, the team of Anca Macovei of the International Rice Research Institute in the Philippines induced mutations in eIF4G using the CRISPR-Cas9 system in the RTSV‐susceptible variety IR64.
The mutation rates ranged from 36.0 to 86.6% and the mutations were successfully transmitted to succeeding generations. Among the mutated eIF4G alleles examined, only those resulting in mutations in SVLFPNLAGKS residues conferred resistance. From these, the team developed lines with RTSV resistance and enhanced yield under greenhouse conditions. These developed lines no longer contain the Cas9 sequence.
These CRISPR-developed RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more resistant rice varieties.
For more information, read the article in Plant Biotechnology Journal.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Researchers Identify Cells that Trigger Flowering
- Scientists Remove 'Biomolecular Brakes' on Plant Oil Production
- Study on Perceptions of Brazilian Farmers Regarding GM Crops
- Experts Confirm Hybridization of Mega-pests in Brazil
- Newly Discovered Hormone Helps Keep Plants from Dehydration
- New Compounds that Keep Plants Fresh
- Collaboration Explores Nature-Friendly Plant Vaccines Against Pests and Diseases
- 'Well Fed' Documentary Wipes Away Fear of GMOs
- Discovery of Gene Controlling Shape and Size of Wheat Spikelets Could Shape Future Crops
- Overexpression of OsTPS19 Confers Enhanced Rice Blast Resistance
- Tomato Transcription Factor Influences Lateral Bud Formation in Tomato
- IBS 2018
- Asian Short Course on Agribiotechnology, Biosafety Regulations and Communication
- Online Courses on Biosafety and Biotechnology
- Researchers Test DNA-Free CRISPR-Editing in Potato
- CRISPR-Cas9 Helps Pinpoint Plant Architecture Genes in Tobacco
- CRISPR-Knock out of FAE1 Gene Improves Camelina Seed Oil Quality
- Tungro-Resistant Rice Developed through CRISPR-Cas9
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: