Scientists Describe Protocol for Multi-gene Genome Editing in MaizeJuly 29, 2020
University of Massachusetts Amherst scientists developed an efficient assembly of large multiplex CRISPR-Cas9 guide arrays for genome editing of maize. Details of the protocol are published in Bio-Protocol.
CRISPR-Cas9 has been known as an effective tool for genome editing, which makes many researchers wishes to use the tool for targeting multiple genes. To address this, researchers developed vectors that target multiple genomic loci from a single transformation event. One of the proposed systems is called MoClo, which is an elaboration of Golden Gate cloning and is particularly fit for assembling larger multiplexed Cas9 guide arrays.
Jarret Man and Madelaine Bartlett described the steps for designing and building a custom guide array targeting any number of maize loci using the MoClo standard components and syntax. Instructions for using variations of the maize and rice U6 to drive guide RNA expression were also provided.
Read the details of the procotol in Bio-Protocol.
You might also like:
- Researchers Use Virus in Wheat and Maize Genome Editing
- Gene Promoter Used to Optimize CRISPR for Targeted Genome Editing in Maize
- CRISPR Nucleases Evaluated for Genome Editing in Maize
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Moderna's COVID-19 Vaccine Advances to Phase 3 COVE Study
- Study: Heartburn Medicine Doesn't Work as COVID-19 Antiviral
- International Team of Researchers Identify Evolutionary Origins of SARS-CoV-2
News from Around the World
- ISAAA Video Shows How to Genetically Engineer a Plant
- Cuba Passes Law to Create Regulating Commission for the Use of GMOs
- German Researchers Decode European Maize Genome
- Gene Switches for Height Identified in Plants
Plant Breeding Innovations
- UC Davis Scientists Use CRISPR Technology to Develop Bull that Produces 75% Male Offspring
- CRISPR-Cas9 Sheds Light on Spikelet Development in Rice
- Scientists Describe Protocol for Multi-gene Genome Editing in Maize
- 132 Research Institutes and Associations Urge the EU to Reconsider Stance on Genome Editing
Read the latest:
- Crop Biotech Update (September 21, 2022)
- Genome Editing Supplement (September 21, 2022)
- Gene Drive Supplement (August 31, 2022)
Subscribe to CBU: