GA20ox-2 Targeted Mutation Produces Semi-dwarf RiceJune 3, 2020
Scientists from the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) reported the targeted mutation of the GA20ox-2 gene using the CRISPR-Cas9 system which led to the development of semi-dwarf rice plants. The findings are published in IOP Science.
GA20ox-2 is a gene encoding an oxidase enzyme involved in the biosynthesis of gibberellin and linked to sd1 locus. In a previous investigation, it was found that the mutation of this gene leads to shorter plants due to defects in the gibberellin signaling pathway. Thus, ICABIOGRAD researchers used CRISPR-Cas9 for targeted mutation of the gene in rice with the expression of two guide RNAs (gRNAs).
The team introduced a single plasmid vector of the CRISPR-Cas9 system harboring dual gRNAs to modify OsGA20ox-2 gene in rice cultivar Kitaake through Agrobacterium-mediated transformation. Results showed a mutation efficiency of 90%. Half of the mutated lines had mutations in both OsGA20ox-2 gRNAs. They observed diallelic mutation with 44 base pair deletions, while three lines were heterozygous, one line was homo-diallelic with 2 base pair insertion, and one line had no mutation. The mutated rice lines exhibited semi-dwarfism, which confirms the disruption of OsGA20ox-2.
Read the research article for more details.
You might also like:
- Semi-dwarf Rice Developed by CRISPR-Cas9-directed Mutagenesis of OsGA20ox2
- Rice Scientists Use CRISPR-Cas9 to Develop High-yielding Semi-dwarf Rice Lines
- Wild Grass to Help in Wheat and Barley Improvement
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Scientists Find Potential Targets for COVID-19 Vaccine
- First Results from Human COVID-19 Immunology Study Identify Universally Effective Antibodies
News from Around the World
- Zambia to Release 2 Drought Tolerant Cowpea Varieties
- Genome Editing and Its Applications in Health and Agriculture
- Chile Serves as a Key Enabler for Global Plant Breeding Innovations
- Omega-3 Canola Oil to Help Reduce Overfishing
- Bt Brinjal Farmers Earned 21.7% More, Satisfied with Yield and Crop Quality
- Plant Science Discovery Uncovers Ways to Improve Immune Responses
- Iranian GM Rice Found to Contribute to Environmental Health
Plant Breeding Innovations
- GA20ox-2 Targeted Mutation Produces Semi-dwarf Rice
Subscribe to CBU: