Molecular Functions of Os-Sc106 Spliceosomal Protein Revealed Using CRISPR-Cas9December 11, 2019
Plants use complicated molecular mechanisms to refine their reactions to various stresses. Their cellular responses affect the expression of genes through regulating processes like transcription and splicing. To explore more on this, Abdulrahman Alhabsi of King Abdullah University of Science and Technology (KAUST) targeted the rice Os-Sc106 locus at exon1, and 3 using CRISPR-Cas9, which is an excellent system to conduct functional knockout studies of genes in most biological systems including plants.
Results of the genotyping analyses revealed the recovery of Os-Sc106 mutants including complete functional knockouts such as sf11h-2, sf11h-8, and sf11h-55. Furthermore, phenotypic analyses showed that Os-Sc106 mutants (sf11h-2, 8, 55, and 57) are oversensitive under abiotic stress in comparison to wild type plants, implying that Os-Sc106 locus encodes a protein that is important for regulating plant stress responses.
Read more from the KAUST University Library.
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