Plasmid-Free Genome Editing of Cabbage and Chinese CabbageNovember 7, 2018
CRISPR-Cas9-mediated gene editing is enabled by the same methods as the production of genetically modified organisms. The only difference is that the transferred DNA sequence is sorted out of the plant at the end of the experiment, making the plant transgene-free. However, researcher Roman Jerala of National Institute of Chemistry in Slovenia and colleagues say that the insertion of the transgene at the earlier part of the experiment can cause unwanted mutations. Also, further expression of the transgene in the plant can cause off-target mutations. Therefore, the team proposed a method to skip transgene production in gene editing.
The team transformed Cas9 enzymes and guide RNA sequence with ribonucleoprotein into plant protoplasts using PEG-mediated transformation. Results showed up to 25 percent mutation frequency in genes FRI and PDS in the said species. They also observed positive correlation between amount of CRISPR components and mutation rate. The team aims to target other genes and study edited protoplast regeneration in the future.
For more information, read the article in Frontiers in Plant Science.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- 13 WTO Members Support Policy Approaches to Enable Innovation in Agriculture
- Farmers and University Students Call for Urgent Action on Biotech Legislation in Uganda
- US Patent Awarded to DNA-Targeting Complex
- High Temperatures Can Trigger a Reaction in a Plant's RNA
- ASTA Lauds International Statement Supporting of Plant Breeding Innovation
- Scientists Discover Gene Regulator that Allows Plant to Rehydrate After Drought
- FAS Jakarta Launches "Biotech Ambassadors" Outreach
- USDA: The Philippines Remains as Asia's Leader in Biotech
- Gene Discovery Could Pave Way for Disease Resistant Crops
- Small Genetic Differences Make Plants into Better Teams
- Agriculture Minister of The Netherlands Opens Door to Genetic Modification
- Protoplast Isolation Method for Genetic Improvement of Pineapple
- Gene from Castor Bean Increases Unusual Oil in Camelina
Beyond Crop Biotech
- Scientists Reveal a Gene Related to Alzheimer's Disease Using CRISPR
- Pocket K: Contributions of Agri-biotech in Alleviation of Poverty and Hunger (Updated)
- Researchers Use CRISPR for Apple and Grapevine Improvement
- Plasmid-Free Genome Editing of Cabbage and Chinese Cabbage
- Plant Genome Editing Database (PGED) Goes Live
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: