Biotech Updates

Scientists Explore Enzyme Recruitment and Mimicry in the CRISPR System

October 31, 2018

The CRISPR system is divided into two classes (I and II), in which class II is the basis for the famous CRISPR-Cas9 system used in several experiments published today. This class, however, is not abundant in nature, covering only 10 percent of the system in sequenced bacteria and archaea. Class I is the more abundant type of the system, but the mechanism of enzyme recruitment during immunity is less studied.

Scientist MaryClare Rollins of Montana State University and colleagues used cryo-electron microscopy to determine the mechanism of Cas enzyme recruitment in the CRISPR system in bacteria. Results showed that a 180 degree shift of a part of the system is involved when the system is bound to the double-stranded DNA. They also found that the complex is mimicked by an "anti-CRISPR," indicating that the class I system may be involved in the evolution of these anti-CRISPRs.

For details, read the article in bioRxiv