Researchers Develop CRISPR-Cas9 Editing Techniques for Ralstonia eutrophaJuly 25, 2018
Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoate (PHA) synthesis and CO2 fixation, making it a potential strain for industrial PHA production and CO2 conversion. However, current methods for genome editing the microbe are inefficient. Thus, developing an efficient method for R. eutropha genome editing is imperative.
The team of Bin Xiong from the University of Chinese Academy of Sciences developed a method for R. eutropha using an electroporation-based CRISPR-Cas9 technique. Using the new method, five R. eutropha genes were successfully edited via homologous recombination, with efficiencies ranging from 78.3 to 100%. The team also found that fructose can reduce the expression of the arabinose-inducible pBAD promoter, which was used to optimize the expression of cas9.
The CRISPR-Cpf1 system and the non-homologous end joining mechanism were also investigated, but failed to yield edited strains.
This study presents the first genome editing method for R. eutropha using an electroporation-based CRISPR-Cas9 approach. This will facilitate more studies and applications of R. eutropha for PHA production and CO2 conversion.
For more information, read the article in Biotechnology for Biofuels.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Society Needs Radical Adaptation to Attain Nutritional Needs in 2050
- Global Biotech Seeds Market to Grow by 9.87% in 2022
- USDA APHIS Deregulates Herbicide Resistant GE Cotton
- Purdue Study Finds Key to Plant Growth Mechanism
- Iran Starts Labeling GM Food
- Filipino Farmers Losing PHP33.85 Billion Due to Delays in Commercializing Bt Eggplant
- Public Participates in Golden Rice Consultations in PH
- Scientists Restrict Bt Gene Expression in Insect Biting Sites
- Researchers Find New Candidate Genes for Disease Resistance in Tomato
Beyond Crop Biotech
- PsFAD3 Gene Involved in ALA Accumulation in Tree Peonies
- Researchers Develop CRISPR-Cas9 Editing Techniques for Ralstonia eutropha
- Plant Genomics & Gene Editing Congress: USA
- Randy A. Hautea Memorial Fund
- Report Identifies 5 Breakthoughs to Advance Food & Agricultural Research by 2030
- Food Matters: Food Security and The Future Of Food
- CRISPR-Edited Tomatoes with Reduced Ethylene Production
- Gene Controlling Megaspore Mitosis in Arabidopsis Found
- FZP Controls Sterile Lemma in Rice Grains
- Organizations, Scientific Leaders Send Open Letter to EC President about Plant Genome Editing
Read the latest:
- Biotech Updates (March 22, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: