Scientists Restrict Bt Gene Expression in Insect Biting SitesJuly 25, 2018
Most of the Bt cotton varieties express Bt genes in all whole-plant tissues. Scientists from Ankara University in Turkey and partners developed a triple-gene construct for the confined expression of GNA, cry1Ac, and cp4 epsps genes to targeted insect biting sites instead of whole-plant tissues. They cloned it at downstream of a wound-inducible promoter isolated from Asparagus officinalis (AoPR1). Furthermore, to widen the range of resistance, GNA was driven by the 35S promoter to target the sap-sucking insects such as aphids which impose large losses in cotton production. To select the transformants in selection medium and for glyphosate tolerance, cp4 epsps gene was included with GNA and cry1Ac genes.
Results showed that the introduction of cry1Ac and GNA to cotton plant conferred resistance to Spodoptera littoralis (African cotton leafworm) and Aphis gossypii Glover (cotton aphid). The results indicate that the restriction of cry1Ac protein to insect biting sites along with a plant lectin attributes significantly contribute to insect pest management strategies.
Read the research article in Plant Biotechnology Reports.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Society Needs Radical Adaptation to Attain Nutritional Needs in 2050
- Global Biotech Seeds Market to Grow by 9.87% in 2022
- USDA APHIS Deregulates Herbicide Resistant GE Cotton
- Purdue Study Finds Key to Plant Growth Mechanism
- Iran Starts Labeling GM Food
- Filipino Farmers Losing PHP33.85 Billion Due to Delays in Commercializing Bt Eggplant
- Public Participates in Golden Rice Consultations in PH
- Scientists Restrict Bt Gene Expression in Insect Biting Sites
- Researchers Find New Candidate Genes for Disease Resistance in Tomato
Plant Breeding Innovations
- CRISPR-Edited Tomatoes with Reduced Ethylene Production
- Gene Controlling Megaspore Mitosis in Arabidopsis Found
- FZP Controls Sterile Lemma in Rice Grains
- Organizations, Scientific Leaders Send Open Letter to EC President about Plant Genome Editing
Beyond Crop Biotech
- PsFAD3 Gene Involved in ALA Accumulation in Tree Peonies
- Researchers Develop CRISPR-Cas9 Editing Techniques for Ralstonia eutropha
- Plant Genomics & Gene Editing Congress: USA
- Randy A. Hautea Memorial Fund
- Report Identifies 5 Breakthoughs to Advance Food & Agricultural Research by 2030
- Food Matters: Food Security and The Future Of Food
Read the latest:
- Crop Biotech Update (June 29, 2022)
- Genome Editing Supplement (June 22, 2022)
- Gene Drive Supplement (June 29, 2022)
Subscribe to CBU: