OsSLA4 Plays a Role in Chloroplast Development in RiceJune 13, 2018
In land plants, chloroplast transcripts undergo post-transcriptional modifications, including splicing, editing, trimming, before translation. This essential step is regulated by a set of nuclear-encoded proteins. Zhong-wei Wang from the China National Rice Research Institute characterized a rice (Oryza sativa) seedling-lethal albino mutant, sla4.
The sla4 mutant exhibited an albino phenotype from germination through the third-leaf stage, and then gradually died. The sla4 mutants also lacked photosynthetic pigments and had severe defects in photosynthesis and early chloroplast development. Analysis showed that a 13-base pair deletion in the coding region of OsSLA4 on chromosome 7 resulted in the albino phenotype. The albino mutants were also generated by knocking-out OsSLA4 using the CRISPR-Cas9 system. OsSLA4 was found to encode a chloroplast-localized pentatricopeptide repeat (PPR) protein.
Loss-of-function of OsSLA4 resulted in severe defects in the intron splicing of several genes as well as a significant reduction in the transcript levels of chloroplast ribosomal RNAs and some chloroplast development- and photosynthesis-related genes.
These results indicate that OsSLA4 is important for early chloroplast development and seedling growth in rice.
For more information, read the article in Plant Growth Regulation.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO Lists 20 Tools for Transforming Food and Agriculture to Achieve SDGs
- Kenya Starts Planting Biotech Cotton Under National Performance Trials
- USDA APHIS Seeks Comments to Deregulate GE Cotton
- Marketing Experts Study Why Consumers Don't See the Benefits of GM Foods
- SABC & ICRISAT Discuss PPP in Agricultural Research, Technology, and Innovation in India
- Plants Help Offspring by Passing on Seasonal Clues
- Speeding Up Photorespiration Boosts Crop Production by 47%
- Genetically Engineered Rice Transports Micronutrients More Efficiently
- PvTRX1h Gene Involved in Regulation of Nodules in Common Bean
- TaPSTOL Controls Agronomically Important Traits in Wheat
Plant Breeding Innovations
- Use of Tomato Promoter Increases CRISPR-Cas9 Efficiency
- OsSLA4 Plays a Role in Chloroplast Development in Rice
- CRISPR Confirms Candidate Gene for Flag Leaf Size in Rice
- Researchers Find Potential Fusarium Wilt Resistance Gene in Tomato
Beyond Crop Biotech
- Kenyan Stakeholders Advocate Harnessing Innovations in Biotech to Curb Plastic Pollution
- AU Launches Report on Emerging Technologies Expected to Spur Africa Development
- 3rd World Biotechnology Congress
- Bt Eggplant Videos
Subscribe to CBU: