Use of Tomato Promoter Increases CRISPR-Cas9 EfficiencyJune 13, 2018
Several expression systems for multiple gRNAs have been developed in the CRISPR-Cas9 system to induce multiple-gene modifications in plants. The team of Ryosuke Hashimoto from Tokushima University in Japan evaluated the mutation efficiencies in the tomato genome using multiplex CRISPR-Cas9 vectors consisting of various Cas9 expression promoters with multiple gRNA expression combinations.
In tomato calli induced with these vectors, mutation patterns varied depending on the promoters used to express Cas9. Notably, a high efficiency of mutations was achieved in the tomato genome when the Cas9 was driven by the tomato ELONGATION FACTOR-1α (SlEF1α) promoter.
These results suggest that optimizing the Cas9 expression promoter used in CRISPR-Cas9 can improve multiplex genome editing.
For more information, read the article in Frontiers in Plant Science.
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