Researchers Find Potential Fusarium Wilt Resistance Gene in Tomato
June 13, 2018| |
The reduced mycorrhizal colonization (rmc) tomato mutant is unable to form mycorrhiza and is more susceptible to Fusarium wilt. The rmc mutant has a chromosomal deletion affecting five genes, one of which is similar to CYCLOPS. Loss of this gene is the cause for non-mycorrhizality in rmc, but not the enhanced Fusarium wilt susceptibility.
The team of Cahya Prihatna from the University of Western Australia studied a second gene in the rmc deletion, designated Solyc08g075770, commonly expressed in roots. Analyses show that Solyc08g075770 encodes a small transmembrane protein with phosphorylation and glycosylation sites. It is predicted to function in transmembrane ion transport and/or as a cell surface receptor.
CRISPR-Cas9 Solyc08g075770 knockout mutants exhibited Fusarium wilt susceptibility like rmc, suggesting that the tomato Solyc08g075770 functions in Fusarium wilt tolerance.
This is the first study to show that Solyc08g075770 is a contributor to the tolerance to Fusarium wilt which was lost in the rmc mutant.
For more information, read the article in Frontiers in Microbiology.
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