Biotech Updates

Researchers Find Potential Fusarium Wilt Resistance Gene in Tomato

June 13, 2018

The reduced mycorrhizal colonization (rmc) tomato mutant is unable to form mycorrhiza and is more susceptible to Fusarium wilt. The rmc mutant has a chromosomal deletion affecting five genes, one of which is similar to CYCLOPS. Loss of this gene is the cause for non-mycorrhizality in rmc, but not the enhanced Fusarium wilt susceptibility.

The team of Cahya Prihatna from the University of Western Australia studied a second gene in the rmc deletion, designated Solyc08g075770, commonly expressed in roots. Analyses show that Solyc08g075770 encodes a small transmembrane protein with phosphorylation and glycosylation sites. It is predicted to function in transmembrane ion transport and/or as a cell surface receptor.

CRISPR-Cas9 Solyc08g075770 knockout mutants exhibited Fusarium wilt susceptibility like rmc, suggesting that the tomato Solyc08g075770 functions in Fusarium wilt tolerance.

This is the first study to show that Solyc08g075770 is a contributor to the tolerance to Fusarium wilt which was lost in the rmc mutant.

For more information, read the article in Frontiers in Microbiology.