ZFN-Mediated Editing Leads to Imidazolinone Herbicide Tolerance in Wheat
May 16, 2018| |
Sequence‐specific nucleases have been used to engineer targeted genome modifications in various plants. While targeted gene knockouts resulting in loss-of-function have been reported, targeted gene editing using DNA repair template from another organism is still challenging.
Researchers from AgriBio in Australia used zinc finger nuclease (ZFN)-mediated editing of a native gene in allohexaploid bread wheat to introduce a specific single amino acid change in the acetohydroxyacid synthase (AHAS) gene using a DNA repair template.
The team recovered edited wheat plants with the targeted modification in one or more AHAS alleles using selection for resistance to Imazamox, an AHAS‐inhibiting imidazolinone herbicide. All ZFN‐mediated mutations were also transmitted to the following generations.
For more information, read the article in Plant Biotechnology Journal.
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