Japanese Researchers Establish New Cas9 VariantMay 2, 2018
Target-AID is a synthetic complex of Cas9 that is paired to an activation-induced cytidine deaminase (AID), which enables targeted nucleotide substitution (C to T or G to A). In a previous study, the team of Zenpei Shimatani from Kobe University in Japan reported the development of herbicide tolerant rice (Oryza sativa) callus via introduction of desired point mutations into target genes through Target-AID.
In this study, the team aimed to establish Target-AID as a plant breeding technique by proving that the introduced mutations in plants regenerated from the transformed callus can be inherited to subsequent generations. Analysis revealed that the regenerated plants as well as their progenies inherited the induced mutations, leading to a generation of selectable marker-free (SMF) herbicide tolerant rice plants.
These findings demonstrate that Target-AID can be developed into novel plant breeding technology, which enables improvement of traits and can be used in combination with targeted base editing systems such as the CRISPR-Cas9 system.
For more information, read the article in Plant Physiology and Biochemistry.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Borlaug Global Rust Initiative Honors Women in Wheat Research
- WEMA Maize Shows Promising Resistance to Fall Armyworm in Mozambique
- Researchers Move Toward Better Understanding of Deadly Citrus Disease
- Cotton Research Goes to Space
- Research Unlocks Rice Gene Diversity for Food Security
- Plant Scientists Boost Malaria Drug Yield in Plant
- Why Grass Cereals Are More Drought-tolerant Than Other Plants
- Europe's Current Approach to Food, Agriculture, and the Environment Not Sustainable, say European National Science Academies
- SlAGL6 Gene is Key to Parthenocarpy in Tomato
- PcWRKY33 Gene from Japanese Knotweed Reduces Salt Tolerance in Transgenic Arabidopsis
- Gene from Wild Peanut Enhances Biotic and Abiotic Resistance in Tobacco
Plant Breeding Innovations
- Wageningen University & Research Releases Booklet on New Plant Breeding Techniques
- CRISPR-Knockout of OsFAD2-1 Gene Improves Rice Bran Oil Health Benefits
- Japanese Researchers Establish New Cas9 Variant
- Early Leaf Senecence and Salt Stress Response Gene Found in Rice
Beyond Crop Biotech
- The Effect of Single Metal Nanoparticles on Transformation Efficiency of Soybean
- FDA Approves Application for AquaBounty Salmon Facility in Indiana, USA
- World Biotechnology Conference
Subscribe to CBU: