The Effect of Single Metal Nanoparticles on Transformation Efficiency of Soybean
May 2, 2018| |
In Vietnam, the efficiency of soybean genetic transformation in DT22 variety remains relatively low at <1%. A study showed for the first time that the efficiency of EHA101 Agrobacterium-mediated genetic transformation in soybean had been remarkably improved with the addition of single metal nanoparticles in shoot induction and shoot elongation media.
The transformation with bar gene conferring herbicide resistance in more than 3,000 half-seeds resulted in a total of 29 transgenic lines which were confirmed by Basta spray 100 mg/l (herbicide containing 24.5% glufosinate) and PCR analysis. The transformation efficiencies were higher with the addition of nanoparticle at 1.6% (Ag), 0.8% (Cu) and 0.8% (Co) compared to the control (0.67%) and Ag nanoparticles showed the strongest effects.
More details from the article in Vietnamese at Tap Chi Nong Nghiep.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Borlaug Global Rust Initiative Honors Women in Wheat Research
- WEMA Maize Shows Promising Resistance to Fall Armyworm in Mozambique
- Researchers Move Toward Better Understanding of Deadly Citrus Disease
- Cotton Research Goes to Space
- Research Unlocks Rice Gene Diversity for Food Security
- Plant Scientists Boost Malaria Drug Yield in Plant
- Why Grass Cereals Are More Drought-tolerant Than Other Plants
- Europe's Current Approach to Food, Agriculture, and the Environment Not Sustainable, say European National Science Academies
-
Research Highlights
- SlAGL6 Gene is Key to Parthenocarpy in Tomato
- PcWRKY33 Gene from Japanese Knotweed Reduces Salt Tolerance in Transgenic Arabidopsis
- Gene from Wild Peanut Enhances Biotic and Abiotic Resistance in Tobacco
-
Beyond Crop Biotech
- The Effect of Single Metal Nanoparticles on Transformation Efficiency of Soybean
- FDA Approves Application for AquaBounty Salmon Facility in Indiana, USA
-
Announcements
- World Biotechnology Conference
-
Plant
- Wageningen University & Research Releases Booklet on New Plant Breeding Techniques
- CRISPR-Knockout of OsFAD2-1 Gene Improves Rice Bran Oil Health Benefits
- Japanese Researchers Establish New Cas9 Variant
- Early Leaf Senecence and Salt Stress Response Gene Found in Rice
-
Read the latest: - Biotech Updates (July 9, 2025)
- Gene Editing Supplement (June 25, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet