Transgenerational CRISPR-Cas9 Induces Multiplex Gene Editing in WheatFebruary 28, 2018
The CRISPR-Cas9-based multiplex gene editing (MGE) can be a powerful method to modify multiple genomic regions simultaneously and control different agronomic traits in crops. In this study, the research team led by Wang Wei from Kansas State University aimed to apply MGE on wheat. The team targeted the TaGW2, TaLpx-1, and TaMLO genes of hexaploid wheat.
The knockout mutations generated in all three copies of TaGW2 resulted in an increase in seed size and thousand grain weight. The team also found that the non-modified targets in early generation plants can still be edited using the same CRISPR-Cas9 construct in the following T generations.
These results demonstrate that transgenerational gene editing can facilitate multiplex gene editing in wheat and can also serve as the source of variation in CRISPR-edited plants.
For more information, read the article in The CRISPR Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- WEMA Gives Hope for Farmers Battling Armyworm in Kenya
- Ghana CSIR Affirms Safety of GM Crops
- Research Reveals New Approach to Improve Nitrogen Use, Enhance Yield, and Promote Flowering in Rice
- Researchers Monitor Photosynthesis in Soybean Using Invisible Light
- Scientists Discover Secret of How to Triple Number of Sorghum Grains
- India Launches Massive Pink Bollworm Awareness Campaign
- Increasing Sugar Yield and Biomass Production in Arabidopsis Discovered by VIB Scientists
- Land Plants Evolved 100 Million Years Earlier than Thought
- Study Shows How Plants Use 'Baits' to Trap Pathogens
- RNAi-mediated Knockdown of GmFAD2-1B Improves Oil Quality in Soybean
- Rice Gene Confers Citrus Canker Resistance to Transgenic Mandarin
Plant Breeding Innovations
- CRISPR-Cas9 Used in Reverse Genetics Studies of Parasponia
- lncRNA1459 Knock-out Alters Tomato Fruit Ripening
- Researchers Apply CRISPR-Cas9 on Cacao Leaves
- Transgenerational CRISPR-Cas9 Induces Multiplex Gene Editing in Wheat
Beyond Crop Biotech
- Researchers Unlock Genetic Code of the Brightest and Most Vibrant Colors in Nature
- BIO Asia International Conference
- Plants, Genes, and Agriculture Book
Read the latest:
- Crop Biotech Update (August 17, 2022)
- Genome Editing Supplement (August 10, 2022)
- Gene Drive Supplement (July 27, 2022)
Subscribe to CBU: