CRISPR-Cas9 Used in Reverse Genetics Studies of ParasponiaFebruary 28, 2018
Parasponia represents five tropical tree species in the Cannabeaceae family and is the only plant lineage besides legumes that can produce nitrogen-fixing nodules with rhizobium. Analyses between legumes and Parasponia allow identification of genetic networks controlling this symbiosis. However, these studies are lacking due to the absence of reverse genetic tools for Parasponia.
The team of Arjan van Zeijl from Wageningen University and Research in the Netherlands reported the use of CRISPR-Cas9 mutagenesis on Parasponia andersonii. Using CRISPR, the team mutated four genes, PanHK4, PanEIN2, PanNSP1, and PanNSP2, which control cytokinin, ethylene or strigolactone hormonal networks. These same set of genes is also responsible for essential symbiotic functions in legumes.
Knockout mutants in Panhk4 and Panein2 displayed developmental phenotypes, namely reduced procambium activity in Panhk4 and disturbed sex differentiation in Panein2 mutants. The symbiotic phenotypes of these two mutant lines also differ from those in legumes. In contrast, PanNSP1 and PanNSP2 were found to be essential for nodule formation, a phenotype similar as reported for legumes.
This study presents the application of CRISPR-Cas9 on Parasponia species as well as its use in reverse genetics studies.
For more on this study, read the article in Frontiers in Plant Science.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- WEMA Gives Hope for Farmers Battling Armyworm in Kenya
- Ghana CSIR Affirms Safety of GM Crops
- Research Reveals New Approach to Improve Nitrogen Use, Enhance Yield, and Promote Flowering in Rice
- Researchers Monitor Photosynthesis in Soybean Using Invisible Light
- Scientists Discover Secret of How to Triple Number of Sorghum Grains
- India Launches Massive Pink Bollworm Awareness Campaign
- Increasing Sugar Yield and Biomass Production in Arabidopsis Discovered by VIB Scientists
- Land Plants Evolved 100 Million Years Earlier than Thought
- Study Shows How Plants Use 'Baits' to Trap Pathogens
- RNAi-mediated Knockdown of GmFAD2-1B Improves Oil Quality in Soybean
- Rice Gene Confers Citrus Canker Resistance to Transgenic Mandarin
Beyond Crop Biotech
- Researchers Unlock Genetic Code of the Brightest and Most Vibrant Colors in Nature
- BIO Asia International Conference
- Plants, Genes, and Agriculture Book
- CRISPR-Cas9 Used in Reverse Genetics Studies of Parasponia
- lncRNA1459 Knock-out Alters Tomato Fruit Ripening
- Researchers Apply CRISPR-Cas9 on Cacao Leaves
- Transgenerational CRISPR-Cas9 Induces Multiplex Gene Editing in Wheat
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: