Overexpression of BjHMGS1 Improves Health-Promoting Compounds in Tomato FruitOctober 18, 2017
The 3-Hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS) codes for isoprenoids including phytosterols, which can lower blood cholesterol levels. In a previous study, overexpressing the mustard (Brassica juncea) BjHMGS1 gene in Arabidopsis and tobacco upregulated genes in the sterol biosynthesis and increased its sterol content. The team of Pan Liao of the University of Hong Kong aimed to overexpress the wildtype (HGMS) and mutant (S359A) BjHMGS1 genes in tomato (Solanum lycopersicum).
Overexpression of either gene caused an accumulation of MVA-derived squalene and phytosterols, as well as vitamin E and carotenoids, in tomato. In tomato with the HMGS-overexpressing seedlings, genes associated with the biosyntheses of universal precursors of several compounds, including phytosterols, brassinosteroids, carotenoids, and vitamin E were upregulated. However, the S359A-overexpressing tomato fruits had greater squalene and phytosterol contents over the HMGS-overexpressing plants.
This study demonstrates that manipulation of the BjHMGS1 gene is a potential strategy to simultaneously elevate health-promoting squalene, phytosterols, α-tocopherol, and carotenoids in tomato.
For more on this study, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Global Hunger Index Shows Long-term Decline But Level Remains High
- Golden Rice to Lead the Way in Producing Healthier Rice Varieties
- Biotechnology a Key Part of Uganda's Food Security Strategy
- ITIF, Independent Scientists Call on US Interior Secretary to Reverse Policy Against GM Seeds
- Australian OGTR Receives Application for Commercial Release of GM Safflower
- Found: Gene to Help Hybrid Wheat Breeding
- Amazonian Farmers Discovered Secret to Wild Rice Domestication 4,000 Years Ago
- Overexpression of BjHMGS1 Improves Health-Promoting Compounds in Tomato Fruit
- The Strawberry FaTPK1 Plays a Critical Role in Fruit Quality Formation
Plant Breeding Innovations
- CRISPR-Cas9-Mediated Targeted Mutagenesis in Seedless Grapes
- Discovery of MPK Roles in Rice by Characterizing CRISPR-Edited Mutants
Beyond Crop Biotech
- Scientists Discover a Gene that Makes Fungi Pathogenic
- Zygote injection of CRISPR-Cas9 Modifies the Target Gene without Unwanted Effects in Pigs
- IPBO Fact Series on Cotton and Maize in Africa
- Updated Pocket Ks on Benefits of GM Crops
Subscribe to CBU: