Scientists Develop Hyper Accurate Cas9 for CRISPROctober 4, 2017
Scientists from the University of California Berkeley continue to be ahead of the CRISPR game. Together with researchers at Massachusetts General Hospital and Harvard University, they have designed a more precise version of Cas9, the enzyme that cuts DNA. The result of their study is published in Nature.
The researchers identified a key region called REC3 in the Cas9 protein that governs how accurately CRISPR-Cas9 homes in on a target DNA sequence, and redesigned it with a hyper-accurate gene editor with the lowest level of off-target cutting to date.
"If you mutate certain amino acid residues in REC3, you can tweak the balance between Cas9 on-target activity and improved specificity; we were able to find the sweet spot where there is sufficient activity at the intended target but also a large reduction in off-target events," said Janice Chen, one of the authors of the study and a graduate student in the lab of Jennifer Doudna, who co-invented the CRISPR-Cas9 gene-editing tool.
The hyper-accurate Cas9 or HypaCas9 has shown to retain its on-target efficiency, but is slightly better at discriminating between on- and off-target sites in human cells.
Read more from Berkeley News.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- GM Wheat Used to Make Bread with Less Gluten
- Uganda Biotechnology Research Boosted by New Facility
- OFAB-Kenya Recognizes Excellence in Science Journalism
- Uganda President Calls for GM Crops Adoption in Africa
- Cagayan De Oro Stakeholders Discuss Philippine Biotech and Biosafety Regulations
- Scientists Decode the Genome of Fall Armyworm
- European Seed Association Puts Plant Breeding Innovation at the Heart of a Sustainable EU Agri-food Policy
- Overexpression of OsERF48 Enhances Root Growth and Drought Tolerance in Rice
- MuMADS1 and MaOFP1 Regulate Fruit Qualities in the Tomato Ovate Mutant
Beyond Crop Biotech
- Overexpressed CYP85A3 Confers Enhanced Biomass Production in Transgenic Plants
From the BICs
- Cagayan De Oro Info Officers Learn about Biotech Social Media Campaign
- Conference of Cereal Biotechnology and Breeding
- 20 Years of Bt Maize in Spain
- Application of CRISPR-Cas9 on Allotetraploid Rapeseed
- Scientists Develop Hyper Accurate Cas9 for CRISPR
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: