Application of CRISPR-Cas9 on Allotetraploid Rapeseed
October 4, 2017| |
CRISPR-Cas9 has been a valuable tool for research and has been widely applied to different plant species. However, this method is still not widely used for the allotetraploid rapeseed (Brassica napus), an important oilseed crop. Huazhong Agricultural University researchers, led by Hong Yang, examined the mutation efficiency of the CRISPR-Cas9 method for 12 genes. The team also determined the pattern, specificity and heritability of these gene modifications in B. napus.
The team found that the average mutation frequency for a single-gene targeted sgRNA in the T0 generation is 65.3%. Homozygotes were also readily found in T0 plants. A total of 48.2% of the gene mutations, including homozygotes, bi-alleles, and heterozygotes, were stably inherited in the next generation (T1) without any new mutations or reversions. Moreover, no mutation was found in the off-target sites among the T0 plants.
These results demonstrate that CRISPR-Cas9 can be an efficient tool for creating targeted genome modifications at multiple loci in rapeseed.
For more on this promising study, read the article in Scientific Reports.
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