Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus Using CRISPR-Cas9
November 23, 2016| |
The targeted genome editing technique, CRISPR/Cas9 system, has been widely used to modify genes. Longxiang Wang, from Huazhong Agricultural University in China, and his colleagues aimed to edit genes related to symbiotic nitrogen fixation (SNF) in the legume Lotus japonicus using CRISPR/Cas9.
The team customized an sgRNA targeting SYMRK (symbiosis receptor-like kinase) loci and generated 20 T0 plants, two of them containing biallelic homozygous mutations. Due to the success of the SYMRK modification, the team then designed two sgRNAs targeting three homologous leghemoglobin loci (LjLb1, LjLb2, LjLb3) to test the possibility of generating multiple gene knockouts.
Twenty out of 70 transformed plants exhibited white nodules, with at least two LjLbs disrupted in each plant. Triple mutant knockouts of LjLbs were also obtained by stable transformation using two sgRNAs.
These results demonstrate that the CRISPR/Cas9 system can support efficient editing of single gene or simultaneous editing of multiple genes in Lotus japonicus.
For more information, read the article in Frontiers in Plant Science.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Report Says Developing World Achieves Significant Growth in Biotechnology
- ICAR-CIRCOT Hosts High Level African Delegation Biotech Study Tour in India
- African Delegation Visits Indian Bt Cotton Farmers
- Scientists Shut off Plant's Sunshield to Boost Productivity
- U.S. Court Rejects GMO Ban in Hawaii County
- NZ Scientists Develop GE Ryegrass for Healthier Animals
- PH Highlights Role of Biotech in National Development
- Genome Sequence of 5,310-Year-Old Maize Cob Provides Insights on Its Early Domestication
-
Research Highlights
- OsACOS12 is Vital in Pollen Exine Formation and Anther Development in Rice
- Overexpression of RAG2 Improves Grain Yield and Quality of Rice
-
Beyond Crop Biotech
- Chinese Scientists Test CRISPR Gene-editing in Human for the First Time
- DBT Prepares India for "Brucella-Free Villages"
-
From the BICs
- Filipino Senior High School Students Undertake Agri-biotech Boot Camp
-
Plant
- Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus Using CRISPR-Cas9
- Researchers Engineer Potyvirus Resistance in Arabidopsis Using CRISPR/Cas9
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet