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Crop Biotech Update

Fungus Genome Assembled Using Nanopore-Generated Reads

November 9, 2016

Researchers from Keygene have assembled the genome of the fungus Rhizoctonia solani using only nanopore-generated reads. Keygene scientists led by Martin de Vos used Oxford Nanopore Technologies' MinIon to generate a 54 megabase genome sequence for R. solani, a soil-borne pathogen causing diseases in major crops including maize, rice, and soybean.

The Oxford Nanopore MinIon produced 77,800 2D pass reads, translated to 834 megabases, and an average read length of 10.7 kilobases. The new assembly is the most contiguous R. solani assembly, according to the researchers. It is also the largest published genome assembly from only nanopore reads.

For more, read the preprint available at BioRxiv.