Targeted Mutagenesis of P450 Gene Confers Male Sterility in MonocotsOctober 26, 2016
Targeted mutagenesis using programmable DNA endonucleases, such as CRISPR and TALENs, is promising in studying gene function in plants and in improving crop yields. Recently, a method that eliminates the need to emasculate the female inbred during hybrid seed production has been described. The system relies on identifying genes critical to anther and pollen development, such as the P450 fertility gene in maize, known as Ms26. However, theP450 gene has not been isolated in other monocots.
The team led by A. Mark Cigan from DuPont Pioneer designed and used a homing endonuclease, Ems26+, to generate in planta mutations in rice, sorghum and wheat orthologs of maize Ms26. Just like in maize, mutations in Ms26 ortholog genes in rice and sorghum prevent pollen formation, resulting in male sterile plants. However, allohexaploid wheat plants that have similar P450 gene mutations in only one of its three genomes were still male fertile.
Targeted mutagenesis and subsequent characterization of male fertility genes in sorghum and wheat could be an important step in improving yields through hybrid seed technology.
For more information, read the full article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- International Team of Scientists Reveals How Plants Turn Light Switch On and Off
- Biofortification Scientists Awarded with 2016 World Food Prize in Des Moines
- Benefits of 20 Years of GE Crops in Canada
- Asian Scientists and Stakeholders Prepare for MOP 8
- New Act Lifts Moratorium on GM Crop Planting in Western Australia
- EFSA Publishes Scientific Opinion for Market Release of GM Cotton GHB119
- Researchers Identify Gene that Influences Plant Root Development
- Scientists Identify Corn Genes in Charge of Calling for Help
- Scientists Analyze Phytoene Synthase 1 Gene Function in Wheat
- Cyanobacterial Flavodoxin Overexpression Confers Abiotic Resistance in Creeping Bentgrass
Plant Breeding Innovations
- Targeted Mutagenesis of P450 Gene Confers Male Sterility in Monocots
Beyond Crop Biotech
- Protein for Treatment of Cocaine Abuse Expressed in Tobacco Leaves
From the BICs
- Pakistan Media Practitioners Trained on Science Communication
- Scientific Conference on GMO Crops
- ISAAA Releases New Pocket K on Marine Biotechnology
Subscribe to CBU: