Metabolic Engineering of Pichia pastoris for the Production of Dammarenediol-II
November 4, 2015| |
Dammarenediol-II is a ginsenoside, active compounds commonly found in Panax species that are of medicinal importance. In previous studies, a dammarenediol-II synthetic pathway was expressed in Pichia pastoris by introducing a dammarenediol-II synthase gene (PgDDS) from Panax ginseng.
To enhance productivity, researchers increased P. pastoris' production of 2,3-oxidosqualene, a precursor of dammarenediol-II. This was done by enhancing the expression of the ERG1 gene, which is responsible for 2,3-oxidosqualene synthesis. This significantly improved the yield of dammarenediol-II over 6.7-fold.
Researchers also reduced the competition for 2,3-oxidosqualene within P. pastoris without its growth. The team downregulated the ERG7 gene, which is responsible for conversion of 2,3-oxidosqualene to lanosterol. The yield of dammarenediol-II was further increased by more than 3.6-fold.
The P. pastoris strains engineered in this study constitute a good platform for further production of ginsenosides in Pichia species.
For further information, read the article in the Journal of Biotechnology.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- AfDB Approves Loan to Promote Science and Technology in Angola
- Scientists Uncover How Plants Thrive Using Natural Mechanism to Recycle Chloroplasts
- BTI Scientists Develop New Technique to Boost Beta-carotene in Tubers
- Pineapple Genome Unlocked; Gives Insight into Photosynthesis in Drought Tolerant Plants
- China's Seed Law Amended
- Australian Researchers Discover Key Gene to Improve Barley's Drought Tolerance
- European Parliament Rejects National GMO Bans Proposal
-
Research Highlights
- GhACL5 Gene is Involved in Stem Elongation and Plant Defense Response to Verticillium dahliae
- AtRAV1 and AtRAV2 Overexpression in Cotton Increases Fiber Length and Delays Flowering under Drought Stress
- Promoter Difference of LcFT1 is the Cause of Natural Variation of Flowering in Litchi
- OsNF-YA7 Transcription Factor Confers Drought Stress Tolerance in Rice
-
Beyond Crop Biotech
- Metabolic Engineering of Pichia pastoris for the Production of Dammarenediol-II
-
From the BICs
- Agri Extension Service Providers in Uganda Hone Biotech Communication Skills
- UBIC Conducts Maiden Forum on Agri-biotech at Bukalasa Agricultural College
-
Resources
- New Pocket K: Coexistence of Biotech and Non-biotech Crops
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet