Biotech Updates

CsCCD7 and CsCCD8 Genes Control Axillary Bud Growth in Saffron Corm

June 25, 2014

Saffron (Crocus sativus) develops new corms, or underground storage stems, at the base of every shoot that grew from the maternal corm. Hence, the number of corms that can be produced from the mother corm is influenced by the number of bud sprouts from it. Strigolactones (SLs) are plant hormones involved in suppression of axillary bud growth. Hence, it was investigated to determine if SLs have any involvement in the corm productivity of saffron. Two of the genes in the SL pathway, CCD7 and CCD8 were focused on and evaluated. These genes encode enzymes required for the production of SL.

The CsCCD7 and CsCCD8 genes were quite similar, but were not identical. CsCCD8 was highly expressed in dormant axillary buds and cutting the apical bud dramatically reduced its expression suggesting its role in the suppression of axillary bud growth from the corm. Furthermore, in vitro experiments showed the involvement of auxin, cytokinin, and jasmonic acid on the sprouting of axillary buds from corms where the apical bud was removed. Results reveal that strigolactones suppress the outgrowth of the axillary buds, similar to its suppression of the above-ground shoot branching.

To learn more on this study, visit http://www.biomedcentral.com/1471-2229/14/171/abstract.