Overexpression of Soybean SAMT gene Confers Resistance to Soybean Cyst Nematode
November 27, 2013| |
Salicylic acid is plays an important function in turning on plant defense mechanisms during pathogen attack. Salicylic acid methyltransferase (SAMT) regulates the amount of salicylic acid by converting salicylic acid to methyl salicylate University of Tennessee scientist Jungyu Lin and colleagues reported that SAMT gene from soybean (GmSAMT1) plays a significant function in the plant's defense against soybean cyst nematode. The researchers isolated full-length cDNAs of GmSAMT1 from a nematode-resistant soybean line and from a nematode-susceptible soybean line. The two cDNAs encode proteins of identical sequences. The GmSAMT1 cDNA was expressed in Escherichia coli. Analysis confirmed that E. coli-expressed GmSAMT1 function as salicylic acid methyltransferase.
To verify the function of GmSAMT1 in soybean defense against soybean cyst nematode, genetically engineered hairy roots overexpressing GmSAMT1 were developed and tested for soybean cyst nematode resistance. Overexpression of GmSAMT1 in nematode-susceptible backgrounds significantly decreased the development of soybean cyst nematode, which implies that overexpression of GmSAMT1 in the GE hairy root system could confer resistance to soybean cyst nematode. It was also found that overexpression of GmSAMT1 in GE hairy roots has an effect in the expression of selected genes involved in salicylic acid production and salicylic acid signal transduction.
Read more results at Plant Biotechnology Journal: http://onlinelibrary.wiley.com/doi/10.1111/pbi.12108/abstract.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Scientists Develop Disease Resistant Pea Lines
- International Team Sequences Fungus Known for Plant Root Symbiosis
- Learning from Burkinabe Farmers' Experiences with Bt Cotton
- Scientists Reveal Historic Footprints of Monkey Flower's Mutation
- Canadian Government Invests in Ug99 Wheat Research
- Ramping Up Phytochemical Pterostilbene in Crops
- Increasing Cropping Frequency to Boost Food Production
- Experts Discover Corn Mutant that can Resist Rootworm Beetles
- Symposia and Cartoon Contest Promote Understanding of Biotechnology in Japan
- Philippine Department of Education Recognizes Key Role of Biotech in National Development
- CAS Academician Highlights Significance of Genetic Science
- Experts Enlighten Malaysia MPs on Biotech
- Chinese Scientists Reveal the Genomic Enigma of Desert Poplar
- A Plant which Acclimatizes with No Exterior Influence
-
Research Highlights
- Scientists Investigate Asian Corn Borer Larvae's Mechanism of Tolerance to Bt Toxins
- Overexpression of Soybean SAMT gene Confers Resistance to Soybean Cyst Nematode
-
Beyond Crop Biotech
- Researchers Produce Campylobacter Genome Map
-
Announcements
- Frontiers in Plant Research - A Workshop for New Plant Scientists
- Fourth International Rice Congress
-
Resources
- Realizing Africa's Rice Promise
-
Read the latest: - Biotech Updates (November 27, 2024)
- Gene Editing Supplement (November 27, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet