Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2022

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	Electrotransfection
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	Multiplex, transfection of one plasmid carrying tandem sgRNA expression cassettes.
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs