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Crop Biotech Update

Researchers Analyze Stress-sensitive Proteins in Broccoli During Post-harvest Aging

July 29, 2011

In a previous study, it was observed that transgenic tobacco that harbored the bacterial isopentenyltransferase (ipt) gene exhibited delayed post-harvest aging. To further investigate this occurrence, Mao-Sen Liu of Academia Sinica in Taiwan and colleagues conducted a proteomic analysis of heads of ipt-transgenic and non-transgenic inbred lines of broccoli at harvest and after four days post-harvest storage.

Results showed that at harvest, there was accumulation of stress-sensitive proteins involved in the maintenance of protein folding, scavenging of reactive oxygen species, and stress protection. Four days after the harvest, the levels of proteins involved in protein folding and carbon fixation decreased significantly, which indicates that cellular degradation and alteration in metabolism have occurred towards senescence.

Based on the findings, the accumulation of stress-sensitive proteins and antioxidant enzyme in ipt-transgenic broccoli are related with delay of post-harvest senescence.

Read the abstract at