Over-expression of Plasma Membrane Protein Gene Enhances Cold-resistance in TobaccoJuly 29, 2011
The plasma membrane is the one of the first structures affected when plants are exposed to low temperatures. To improve low temperature-resistance in plants, Dong-Ru Feng and colleagues at the Sun Yat-sen University in China conducted a study using a cloned cold-induced gene (MpRCI) from plantain.
They used green fluorescent protein fused with MpRCI and traced that the protein product is localized in the plasma membrane. Through real time polymerase chain reaction (RT-PCR), the profile of the gene was analyzed. It was found out that the gene is induced by low temperatures in leaves and leafstalk, but not in the shoot meristem or roots. They also cloned a section of the gene that they predicted to have a number of elements connected with abiotic stresses. Results showed that the sequence has characteristic of low temperature- and abscisic acid influenced activity.
Phenotypic espial and ion leakage assays of transgenic tobacco containing the gene resulted to over-expression of the cold-induced plasma membrane protein gene MpRCI, improving the cold-resistance of the plants.
The findings imply that MpRCI is involved in maintaining the stability of the plasma membrane at low temperatures.
Read the research article featured at the special issue on Temperate Responses in Plants at http://www.sciencedirect.com/science/article/pii/S0098847208001676.
See more articles:
News from Around the World
- Strategies to "Freeze the Footprint of Food"
- Kenya Pushes Through GM Cotton Plans
- Africa Needs to Increase S&T Investment
- USDA Reopens Comment Period for Draft EA for Drought Tolerant Corn
- US EPA Approves Syngenta's Dual Modes of Insect Protection with Five Percent Refuge
- Genomics Institute to Offer Remote Data Service
- CSIRO Researchers Develop Crops for the Changing Climate
- Expert Says GM Risk Communication in China Should Be Strengthened
- Reduced Pesticide Poisoning Observed Among Cotton Growers
- Pakistan Needs to Collaborate with China on Agriculture
- Pakistan Seed Board Approves New Seed Varieties
- Gene Discovery in Wild Barley May Lead to Stress Tolerant Crops
- Second Confined GM Field Tests Approved in Miyazaki University
- GM Crops are Essential Innovation in Japanese Agriculture, says JBA President
- GM Drug Trials Starts in UK
- Field Trials Destroyed in Germany
- Andalusian Farmers and Government Officials Want Biotech Crops
- Barley Defense System Against Powdery Mildew
- Scientists Investigate Effect of Rice Sucrose Transporters on Potato Starch Yield
- Over-expression of Plasma Membrane Protein Gene Enhances Cold-resistance in Tobacco
- Researchers Analyze Stress-sensitive Proteins in Broccoli During Post-harvest Aging
Beyond Crop Biotech
- Molecular Portrait Reveals Cell Signaling Activity
- Purdue Biologists Elucidate Bacterial Infection
- Center for Transgenic Mouse Research
- BIO Releases Second Edition of GE Animals Benefits Study
- Asian Food Security Conference in Singapore
- Food and Nutrition in the 21st Century, Warsaw, Poland
- Biotech Country Facts and Trends
- COMSTECH Website
Subscribe to CBU: