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Gene Replacements and Insertions in Rice via Intron-Targeting CRISPR |
Sequence-specific nucleases have been exploited to create targeted gene knockouts in various plants. However, replacing a fragment and even obtaining gene insertions at specific loci in plant genomes remain challenging. Chinese Academy of Sciences' Jun Li, along with a group of researchers, describes an efficient intron-mediated site-specific gene replacement and insertion that generate mutations using non-homologous end joining (NHEJ) using CRISPR/Cas9.
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A biweekly update on gene editing research, regulations, and impact
produced by ISAAA Inc. |
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| A monthly update on gene drive research and development provided by ISAAA in collaboration with the Outreach Network for Gene Drive Research |
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