OsPP65 Negatively Regulates Osmotic and Salt Stress Responses in Rice
July 6, 2022| |
A team of researchers from Guangdong Academy of Agricultural Sciences reported that rice gene OsPP65 is responsive to multiple stresses and is induced by osmotic and salt stress treatments. Their findings are presented in Rice journal.
Type 2C protein phosphatases (PP2Cs) have vital functions in controlling plant development and various stress responses. However, their specific roles in rice abiotic stress tolerance are yet to be discovered. Thus, the researchers turned off OsPP65 using CRISPR-Cas9 to know more about their functions in rice.
Results showed that OsPP65 knockout rice plants had improved tolerance to osmotic and salt stresses. Significantly higher induction of genes involved in jasmonic acid and abscisic acid biosynthesis or signaling, as well as higher contents of endogenous JA and ABA, were found in the OsPP65 knockout plants compared with the control when exposed to osmotic stress. Further analysis showed that JA and ABA function independently in osmotic stress tolerance due to the loss of OsPP65.
Read the research article in Rice.
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