Biotech Updates

Improved Tolerance toward Fungal Diseases in Transgenic Cavendish Banana

January 28, 2011

A new transformation system was developed by Jane Vishnevetsky of the Agricultural Research Organization in Israel and colleagues to increase banana's resistance to Sigatoka leaf spot disease which is the most devastating fungal disease of banana caused by Mycosphaerella fijiensis. They developed transgenic banana lines with endochitinase gene ThEn-42 from Trichoderma harzianum, stilbene synthase (StSy) gene from grapes, and uperoxide dismutase gene Cu,Zn-SOD from tomato for intense resistance to fungal attack. The lines were subjected to field trials for four years and several banana lines exhibited improved tolerance to the pest. To test if the genes conferring Sigatoka tolerance also provides resistance to other fungal pests, they inoculated gray mould (Botrytis cinerea) to the transgenic plants. Results showed that the lines with best Sigoka tolerance also showed tolerance to gray mould.

Read the abstract at http://www.springerlink.com/content/j258541881648020/.