Biotech Updates

Researchers Develop Rapid Detection of Grass Carp Reovirus (GCRV) Using RPA-based Test Strips and CRISPR-Cas13a System

November 15, 2023

Researchers from the Yangtze and Jilin University in China developed a rapid on-site diagnosis method with high sensitivity, specificity, and on-site practicability for detecting grass carp reovirus (GCRV) type 1 in aquatic animals.

GCRV poses a significant threat to the aquaculture industry. It causes minimal muscle bleeding and severe intestinal bleeding, which greatly contributes to a high mortality rate in grass carp. Currently, there are a number of approaches to detect GCRV, including electron microscopic observation and virus isolation. However, these approaches are time-consuming, not specific, and have low sensitivity to GCRV.

Thus, the researchers combined recombinase polymerase amplification (RPA)-based test strips and CRISPR-Cas13a-based molecular detection platform to create a GCRV detection system. The results of the study show that this method has great potential in effectively monitoring and preventing the spread of the virus in grass carp and in the aquaculture industry.

Read the article from Frontiers in Microbiology for more information.

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