Researchers Develop Rapid Detection of Grass Carp Reovirus (GCRV) Using RPA-based Test Strips and CRISPR-Cas13a System
November 15, 2023| |
Researchers from the Yangtze and Jilin University in China developed a rapid on-site diagnosis method with high sensitivity, specificity, and on-site practicability for detecting grass carp reovirus (GCRV) type 1 in aquatic animals.
GCRV poses a significant threat to the aquaculture industry. It causes minimal muscle bleeding and severe intestinal bleeding, which greatly contributes to a high mortality rate in grass carp. Currently, there are a number of approaches to detect GCRV, including electron microscopic observation and virus isolation. However, these approaches are time-consuming, not specific, and have low sensitivity to GCRV.
Thus, the researchers combined recombinase polymerase amplification (RPA)-based test strips and CRISPR-Cas13a-based molecular detection platform to create a GCRV detection system. The results of the study show that this method has great potential in effectively monitoring and preventing the spread of the virus in grass carp and in the aquaculture industry.
Read the article from Frontiers in Microbiology for more information.
| |
You might also like:
- CRISPR-Cas13a Shows Efficient Phage Genome Editing
- CRISPR-Cas12a-based DNA Test Used in Disease Detection and GMO Identification
- Biotech Can Help Save World’s Aquaculture from Climate Change
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
Plant
- FDA, EPA, and USDA Release New Documents on Biotechnology Regulation
- Researchers Take a Step Closer to Releasing the First Genetically Edited Potato in Latin America
- Royal Society: Evidence-led GM Crop Regulation Could Help Tackle Global Food Security and Climate Change Risks
-
Animal
- Review Discusses Application of DNA Methylation in Poultry
- Researchers Develop Rapid Detection of Grass Carp Reovirus (GCRV) Using RPA-based Test Strips and CRISPR-Cas13a System
- Genetic Tools For Conservation and Health: What's The Role of Gene Drives?
- Pinoy Biotek Seminar: Stock Enhancement of Blue Swimming Crab
-
Health
- Review Highlights Latest In Vitro Models on Neurodegenerative Diseases
-
Environment
- World's First Synthetic Yeast Near Completion
-
Read the latest: - Biotech Updates (July 24, 2024)
- Gene Editing Supplement (July 17, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet