Biotech Updates

Study Reveals Role of Soybean 14-3-3 Gene on White Mold Resistance

December 1, 2022

Researchers from Ottawa Research and Development Centre and partners investigated the role of the soybean 14-3-3 gene Glyma05g29080 on white mold resistance and nodulation using CRISPR-Cas9 editing and RNA silencing. Their findings are published in the journal Molecular Plant-Microbe Interactions.

The 14-3-3 gene family plays a vital role in physiological processes, such as controlling metabolism, hormone signaling, cell division, and responses to various biotic and abiotic stresses. Thus, the research team conducted a loss-of-gene function study with CRISPR-Cas9 and RNAi. Particle bombardment was used to insert CRISPR and target the soybean 14-3-3 gene and an RNAi construct.

Results showed that the transgenic plants and their progeny were more susceptible to Sclerotinia sclerotiorum infection and significantly reduced nodulation. These findings confirm the role of the 14-3-3 gene in both nodulation and defense.

Download the open-access article in Molecular Plant-Microbe Interactions.

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