Double Knockout of OsWRKY36 and OsWRKY102 Boosts Rice LignificationMarch 25, 2020
Experts from Kyoto University and partner institutions developed double mutant lines of rice to gather information on enhancing lignin deposition in grass species which are vital lignocellulose feedstocks. Their findings are reported in Plant Science.
Enriching plant's lignin contributes to improved applications of lignocellulosic biomass into solid biofuels and valuable aromatic chemicals. This led Takuji Miyamoto and colleagues to generate rice transgenic lines deficient in OsWRKY36 and OsWRKY102, which code for putative transcriptional repressors for secondary cell wall formation. They used CRISPR/Cas9-mediated targeted mutagenesis and chemical and nuclear magnetic resonance (NMR) methods to characterize altered cell walls.
The results showed OsWRKY36 and OsWRKY102 mutants had boosted lignin content by up to 28% and 32%, respectively. Furthermore, double mutants had enriched lignin in the cell walls by up to 41%, with substantially altered culm morphology compared to the single mutant lines and wild-type controls.The findings indicate that both OsWRKY36 and OsWRKY102 are involved in the repression of rice lignification.
Read the article in Plant Science for more findings.
You might also like:
- Plant Breeding Innovation: CRISPR-Cas9
- Disrupting Flavone Synthase II Alters Lignin and Improves Biomass Digestibility
- Sandia Studies Enzyme that Breaks Down Lignin Derivatives
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Biotech Crops in the Pipeline to Improve Food Quality and Bring Environmental Benefits
- Local Government Officials Warn Scientists of Biotech Critics in Uganda
- US Proposes to Use Genetically Engineered Crops to Support Southeastern Wildlife Refuges
- Omega-3 Canola Oil Bound for Market by Second Quarter of 2020
- Most Americans Believe GMOs Likely to Improve Global Food Supply
- Russia to Expedite Genetic Technologies and Genome Editing for Public Health, Agriculture, and Industry
- Researchers Develop Gene Disruption Method for Plant Pathogen
- Double Knockout of OsWRKY36 and OsWRKY102 Boosts Rice Lignification
- International Seed Federation Calls on Governments to Facilitate Movement of Seeds in the Time of COVID-19
- Scientists Say COVID-19 Coronavirus Has Natural Origins
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: