Researchers Use Virus in Wheat and Maize Genome Editing
July 10, 2019| |
Scientists from China Agricultural University engineered a barley stripe mosaic virus (BSMV)-based guide RNA delivery system for targeted mutagenesis in wheat and maize. The results are published in Molecular Plant Pathology.
BSMV‐based delivery of single gRNAs for CRISPR-Cas9-mediated targeted mutagenesis was first used in Nicotiana benthamiana, a close relative of tobacco. To test if this can also be applied to economically important crops, the researchers transformed wheat and maize with Cas9 nuclease gene and selected the wheat TaGASR7 and maize ZmTMS5 genes as targets to evaluate the feasibility and efficiency of BSMV‐mediated mutagenesis. Results showed that targeted mutagenesis was successful for wheat and maize with efficiencies reaching 78% and 48%, respectively.
Based on the results, BSMV‐based delivery of single gRNAs could be a useful tool in economically important crops.
Read more results in Molecular Plant Pathology.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Nigeria Needs More Community Engagement on GM Cotton from Agricultural Extension Workers
- Curriculum Reforms in Uganda Integrate Modern Biosciences
- Mozambique to Gain from Using GM Maize, Research Reveals
- Researchers Map Vegetable Family Tree
- Breakthrough Finds Plant Nutrient Detector
- Scientists Discover How Plants Breathe
- Change Needed for Europe's Assessment and Approval Process for Genome‐Edited Crops
-
Research Highlights
- Golden Rice Has Same Nutrients as Traditional Rice Except for the Increased Provitamin A Content
-
Beyond Crop Biotech
- Japanese Geneticists Favor New Policies for Genome Editing Applications, Survey
-
From the BICs
- Project SAFFAL Reaches Farmers Affected by Fall Armyworm in Karnataka
-
Announcements
- 6th Plant Genomics and Gene Editing Congress: Asia (10% Discount for CBU Subscribers)
-
Plant
- EU Commissioner Vytenis Andriukaitis on the Potential of Gene Editing
- Genome Editing Could be Used to Engineer Resistance to Virus and Cold-induced Sweetening in Potato
- Scientists Finally Record Successful Mitochondrial DNA Editing in Plants
- Researchers Use Virus in Wheat and Maize Genome Editing
-
Read the latest: - Biotech Updates (May 24, 2023)
- Gene Editing Supplement (May 24, 2023)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet