Successful Deletion of Rice Retrotransposon Using CRISPR-Cas9May 22, 2019
A successful example of transposon deletion using CRISPR-Cas9 as an alternative technique for plant breeding was presented by experts from National Agriculture and Food Research Organization (Japan) and Yokohama City University. The results of their study are published in Plant Cell Reports.
Studies have shown that transposition of transposable elements (TEs) has an impact on adjacent genes, which can lead to alterations in genetic traits, including expression levels and patterns, splicing and epigenetic status, and function of genes in or close to the added or removed locus may be affected. Intentional alteration of loci adjacent to TEs or TEs themselves using genome editing could mimic the naturally occurring translocation of TEs, and lead to development of novel plants. Thus, the researchers performed targeted deletion of Tos17 retrotransposon, which is flanked at both ends by long terminal repeat (LTR) sequences, through genome editing in rice.
Results showed successful targeted mutagenesis of the LTR, and targeted deletion between LTRs. From the transformed calli, the next generation of the regenerated plants lack Tos17. These results indicate successful deletion of the Tos17 retrotransposon from the rice genome using CRISPR-Cas9.
Read more about the study in Plant Cell Reports.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- University of Michigan Puts Spotlight on Smarter Food Systems to Attain Zero Hunger
- Ethiopian Women in Biosciences Trained on Science Communication
- Software Locates Sugarcane Genes of Interest
- Scientists Use Open-Source Genetic Analysis Method in Plant Cells for the First Time
- Technology to Measure Plant Improvements Helps Boost Production
- US to Help Pakistan Introduce GE Corn
- UC Professor Emerita Emphasizes on Gene Editing to Achieve Sustainability Needs
- Scientists Discover RNA Controlling Tuber Formation in Potatoes
- Iron-rich GM Wheat Set to Undergo Field Trials
- Sweetpotato Enzymes Expressed in Arabidopsis Revealed Additional Functions of VEPs
- Research Shows How Transposon Insertion Confers Resistance to Bt Cotton in Pink Bollworm
Beyond Crop Biotech
- Scientists Sequence Charleston Gray Watermelon
- Experts Use CRISPR-Cpf1 for Cotton Genome Editing
- Successful Deletion of Rice Retrotransposon Using CRISPR-Cas9
Read the latest:
- Biotech Updates (June 7, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: