Scientists Use Open-Source Genetic Analysis Method in Plant Cells for the First TimeMay 22, 2019
Scientists at the U.S. Department of Energy's Lawrence Berkeley National Lab (Berkeley Lab) have successfully used an open-source RNA analysis platform for the first time in an effort that could help engineer more efficient food and biofuel crops.
Drop-seq, developed at Harvard Medical School in 2015, is a popular method for measuring the RNA present in individual cells, allowing scientists to see what genes are being expressed and how this relates to the specific functions of different cell types. However, the freely shared protocol has only been used in animal cells. Diane Dickel, a scientist from Berkeley Lab pointed out that this is important in understanding plant biology. Dickel said that plants, like humans and mice, have multiple cell and tissue types, but unlike animals, plants have cell walls, which make it hard to open the cells up for genetic study.
While Dickel was interested in using Drop-seq in plants, some were skeptical. To run plants cells through a single-cell RNA-seq analysis, they must be protoplasted, a process that will strip their cell walls using enzymes. Dickel and her colleagues at DOE Joint Genome Institute (JGI) teamed up with researchers from UC Davis who had perfected a protoplasting technique for root tissue from Arabidopsis thaliana. After preparing samples of more than 12,000 Arabidopsis root cells, the group was thrilled when the Drop-seq process went smoother than expected.
For more details, read the news release from the Berkeley Lab.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- University of Michigan Puts Spotlight on Smarter Food Systems to Attain Zero Hunger
- Ethiopian Women in Biosciences Trained on Science Communication
- Software Locates Sugarcane Genes of Interest
- Scientists Use Open-Source Genetic Analysis Method in Plant Cells for the First Time
- Technology to Measure Plant Improvements Helps Boost Production
- US to Help Pakistan Introduce GE Corn
- UC Professor Emerita Emphasizes on Gene Editing to Achieve Sustainability Needs
- Scientists Discover RNA Controlling Tuber Formation in Potatoes
- Iron-rich GM Wheat Set to Undergo Field Trials
- Sweetpotato Enzymes Expressed in Arabidopsis Revealed Additional Functions of VEPs
- Research Shows How Transposon Insertion Confers Resistance to Bt Cotton in Pink Bollworm
Plant Breeding Innovations
- Experts Use CRISPR-Cpf1 for Cotton Genome Editing
- Successful Deletion of Rice Retrotransposon Using CRISPR-Cas9
Beyond Crop Biotech
- Scientists Sequence Charleston Gray Watermelon
Read the latest:
- Crop Biotech Update (August 17, 2022)
- Genome Editing Supplement (August 10, 2022)
- Gene Drive Supplement (July 27, 2022)
Subscribe to CBU: